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Histological Staining & Analysis

  • The cellular profile of a wound tissues changes with time after injury

  • Initially, inflammatory cells that are responsible for clearing microbial contamination and wound site debris (e.g. neutrophils & monocyte-macrophages) predominate

  • Later, as the wound heals (matures) proliferative cells - which are responsible for the generation of replacement tissues (e.g. fibroblasts & endothelial cells) proliferate and predominate.

  • As wound healing progresses new extracellular matrix (e.g. collagen) is synthesised and remodelled into scar tissue.

  • Consequently, the cellular profile of a wound can be used to measure wound healing progression.

H&E Histology measures.png
BrDU detection.png
CD31 staining.png
Smooth Muscle Actin 2.png
IBA macrophage staining.png
Collagen staining - PSR detection.png

Cell/Matrix Specific Staining

  • Wound tissues are harvested at time points determined by the agent under test and the purpose of the study

  • Specific staining of key cells/cellular activities is then undertaken e.g.-

    •  neutrophils

    •  macrophages

    •  angiogenesis

    •  proliferation

    •  collagen

    • smooth muscle actin

  • Stained sections are then digitally scanned  & analysed

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