Histology Staining and Analysis

Cellular Profile of Wound Tissues

• The cellular profile of a wound tissues changes with time after injury

• Initially, inflammatory cells that are responsible for clearing microbial contamination and wound site debris (e.g. neutrophils & monocyte-macrophages) predominate

• Later, as the wound heals (matures) proliferative cells - which are responsible for the generation of replacement tissues (e.g. fibroblasts & endothelial cells) proliferate and predominate.

• As wound healing progresses new extracellular matrix (e.g. collagen) is synthesised and remodelled into scar tissue.

• Consequently, the cellular profile of a wound can be used to measure wound healing progression.

  
  

Cell/Matrix Specific Staining

• Wound tissues are harvested at time points determined by the agent under test and the purpose of the study

• Specific staining of key cells/cellular activities is then undertaken e.g.-

  • neutrophils

  • macrophages

  • angiogenesis

  • proliferation

  • collagen

  
  

• Stained sections are then digitally scanned  & analysed

A close-up of an illuminated microscope lens in a lab, emphasizing the precision needed for scientific research.